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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two supplemental splice variants ended up described but thorough nucleotide sequence info is not really nonetheless accessible.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This guideline outlines additional achievable optimization of cellular and immunoassay parameters to make certain the very best effects are...
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This guide outlines even more feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind towards the epitope around the target protein. By evaluating the staining through the blocked antibody compared to the antibody alone, you can see which staining is distinct.
Goal Info Cyclin E belongs to the highly conserved cyclin household, whose users are characterised by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation patterns which add to the temporal coordination of each mitotic occasion. Cyclin E types a fancy with and capabilities for a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S phase boundary and is degraded as cells progress by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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